Fig. 2From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactionsSDS-PAGE analysis of the conjugation reaction between hFasLECD-TCO and mPEG-MTZ. Lanes: M, molecular-weight size markers; 1, before reaction, 2–5, after reaction, (2: 0.5, 3: 1.0, 4: 1.1 and 5: 1.5 M excess amounts of mPEG-MTZ were reacted with hFasLECD-TCO)Back to article page