Table 1 Purification performance of the monomeric and trimeric enzymes
ITC purification | Specific activity(U/mg) | Purification fold | Recovery of activity (%) | |
|---|---|---|---|---|
B-E-F | Crude | 11.9 ± 0.5 | 7.4 | 68.7 ± 2.2 |
Purified | 88.2 ± 3.5 | |||
B-E | Crude | 8.1 ± 0.8 | 4.4 | 56.2 ± 1.4 |
Purified | 35.4 ± 0.9 | |||
X-E-F | Crude | 41.4 ± 0.1 | 12.5 | 66.3 ± 1.4 |
Purified | 516.0 ± 10.3 | |||
X-E | Crude | 45.4 ± 0.1 | 5.8 | 60.2 ± 2.8 |
purified | 265.2 ± 3.9 | |||