Fig. 1

Purification and analyzed the target enzymes by SDS-PAGE and MALDI-TOF. Monomeric enzymes B-E a, b and X-E c, d; trimeric enzymes B-E-F e, f and X-E-F g, h Lane M: marker; lane1 the lysate; lane 2 supernatant of first-round ITC, lane 3 supernatant of second-round ITC