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Table 1 Exemplary companies providing immune repertoire products and services

From: Overview of methodologies for T-cell receptor repertoire analysis

Company Service/Kit Starting material Library preparation approach Chains CDR regions Organism Sequencing platform and length (bp)
BGI (Shenzhen, China) Service gDNA
RNA
Mplex-PCR:
primers V- C genes
TCRαTCRβ
BCRH, BCRL
CDR3 Human Illumina:
HiSeq2000/2500
(100 × 2 bp)
MiSeq
(150/300 × 2 bp)
Roche454
RNA 5’RACE TCRα, TCRβ CDR1
CDR2
CDR3
Adaptive
Biotechnologies-ImmunoSeq
(Seattle, USA)
Service
Kit
gDNA
cDNA
Mplex-PCR:
primers V-J genes
TCRα, TCRβ, TCRδ, TCRγ,
BCRH, BCRL, BCRK
CDR3 Human
Mouse
Illumina:
HiSeq, MiSeq
iRepertoire, Inc.
(Huntsville, USA)
Service
Kit
gDNA Mplex-PCR:
primers V-J genes
TCRβ CDR3 Human Illumina:
HiSeq, MiSeq
(100/150 × 2 bp)
RNA Mplex-PCR:
primers V-C genes
TCRα, TCRβ, TCRδ, TCRγ,
BCRH, BCRL
CDR2
CDR3
Human
Mouse
Illumina:
HiSeq, MiSeq
(100/150/250 × 2 bp)
Roche454 (500 bp)
Clonotech
Takara Bio USA, Inc.
(Mountain View, USA)
Kit RNA SMART technology (5’RACE) TCRα, TCRβ CDR1 CDR2 CDR3 Human
Mouse
Illumina:
HiSeq, MiSeq
Used by company
for validation:
MiSeq (300 × 2 bp)
  1. Many different features are available; these can be combined in more than one way. Choice of primers, sequencing platform and depth may vary depending on starting material and desired outcome. Adaptive Biotechnologies only uses cDNA for limited applications. The company applies primer concentration controls for amplification bias correction and different options for sequencing depth are available (survey, deep, ultra-deep, max depth). iRepertoire may offer sequencing of the CDR2 region, depending on the chosen sequencing length