Fig. 2

HPLC analysis of metabolites from the wild type L. enzymogenes C3 (a), from the transposon mutant 5E4 (b) and from in trans complemented strain (P2) of the clp mutant (5E4) (c). Strain C3 was grown in a medium containing 5 μg/mL chloramphenicol, strain 5E4 and strain P2 were in 50 μg/mL chloramphenicol. The peaks for chloramphenicol (CM), three acyl products of chloramphenicol (1, 2 and 3), and HSAF are indicated with arrows. HR-LCMS also identified minor acyl derivatives (Acyl-CM) in the cultures. Note that acyl derivatives (indicated compound 1, see Fig. 1 for structure) of chloramphenicol were also produced predominantly in the culture of strain P2