Table 1 Plasmids and strains used in this study
From: An in vitro synthetic biosystem based on acetate for production of phloroglucinol
Name | Description |
|---|---|
Stains | |
E. coli K-12 | For amplification of gene acs |
A. pasteurianus | For amplification of gene acs1 and acs2 |
A. calcoaceticus | For amplification of four subunits of gene acc |
Plasmids | |
pET-28a(+) | Kan r oripBR322 lacI q T7p |
pET28a-acs | pET-28a(+) carrying acs from E. coli K-12, Kan r |
pET28a-acs1 | pET-28a(+) carrying acs1 from A. pasteurianus, Kan r |
pET28a-acs2 | pET-28a(+) carrying acs2 from A. pasteurianus, Kan r |
pET28a-accA | pET-28a(+) carrying accA from A. calcoaceticus, Kan r |
pET28a-accB | pET-28a(+) carrying accB from A. calcoaceticus, Kan r |
pET28a-accC | pET-28a(+) carrying accC from A. calcoaceticus, Kan r |
pET28a-accD | pET-28a(+) carrying accD from A. calcoaceticus, Kan r |
pET28a-phlD | pET-28a(+) carrying phlD from P. protegens, Kan r |