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Table 1 Plasmids and strains used in this study

From: An in vitro synthetic biosystem based on acetate for production of phloroglucinol

Name Description
Stains  
E. coli K-12 For amplification of gene acs
A. pasteurianus For amplification of gene acs1 and acs2
A. calcoaceticus For amplification of four subunits of gene acc
Plasmids  
pET-28a(+) Kan r oripBR322 lacI q T7p
pET28a-acs pET-28a(+) carrying acs from E. coli K-12, Kan r
pET28a-acs1 pET-28a(+) carrying acs1 from A. pasteurianus, Kan r
pET28a-acs2 pET-28a(+) carrying acs2 from A. pasteurianus, Kan r
pET28a-accA pET-28a(+) carrying accA from A. calcoaceticus, Kan r
pET28a-accB pET-28a(+) carrying accB from A. calcoaceticus, Kan r
pET28a-accC pET-28a(+) carrying accC from A. calcoaceticus, Kan r
pET28a-accD pET-28a(+) carrying accD from A. calcoaceticus, Kan r
pET28a-phlD pET-28a(+) carrying phlD from P. protegens, Kan r