Fig. 4
From: An in vitro synthetic biosystem based on acetate for production of phloroglucinol
Titration of individual enzyme: a ACS2, each assay mixture included 1.5 μM ACC, 1.5 μM PhlD and different concentration of ACS2. b ACC, each assay mixture included 1.5 μM ACS2, 1.5 μM PhlD and different concentration of ACC. c PhlD, each assay mixture included 1.5 μM ACS2, 1.5 μM ACC and different concentration of PhlD. In all the reactions, substrate and cofactor concentrations were as follows: 10 mM potassium acetate, 30 mM ATP, 3 mM CoA