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Fig. 3 | BMC Biotechnology

Fig. 3

From: An in vitro synthetic biosystem based on acetate for production of phloroglucinol

Fig. 3

Kinetic analysis of acetyl-CoA synthetase. a Enzyme activity comparison of ACS, ACS1, and ACS2. 0.017 nmol (each) of ACS, ACS1, and ACS2 were used to catalyze the reactions at pH 7.4 and 37 °C, respectively. Date represent mean ± standard deviation (N = 3). b Effects of pH on enzymatic activity of His6-tagged ACS2 proteins. The reactions were performed at 37 °C. 100% corresponds to the enzyme activity at the condition of pH7.0 (Tris-HCl). c Effects of temperature on enzymatic activity of His6-tagged ACS2 proteins. The reactions were performed at the condition of pH 7.4 (Tris-HCl). 100% corresponds to the enzyme activity at 37 °C

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