Fig. 6

Daily changes of dose-response curves of ELuc bioluminescence from aflatoxin B1-treated 3D spheroids. The culture medium containing 300 μM D-luciferin and aflatoxin B1 (3.7, 1.9, 0.9, 0.5, and 0.2 μM) was refreshed every 2 to 3 days. 3D cultures and bioluminescence measurement were performed as described in Methods and legend in Fig. 4. The dose-dependent change of bioluminescence intensity was expressed as the percentage of untreated control (set at 100%). Bioluminescence intensity at 50% was marked by a dotted red line. Italic digits in the figures indicate culture day after initial treatment. Error bars indicate standard deviations (n = 4).