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Fig. 1 | BMC Biotechnology

Fig. 1

From: Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Fig. 1

Dose-response curves of luminescence intensity and cytotoxicity in SDS-treated mouse fibroblast A9 cells. ELuc-expressing A9 cells harboring the MI-MAC vector were seeded in 96-well plates and treated with various concentrations of SDS (1000, 500, 250, 125, 63, 31, 16, and 8 μM). After incubation for 48 h in the presence of 200 μM D-luciferin, bioluminescence intensity (green circles) was measured nondestructively, and cell viability (pink circles) was assessed by WST-1 assay using the same cells. Concentration-dependent changes in bioluminescence and cell viability were expressed as percentage of untreated control (set at 100%). Error bars indicate standard deviations (n = 6). Schematic drawing of expression plasmid is shown in the upper panel

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BMC Biotechnology

ISSN: 1472-6750

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