Development of plant-produced protein body vaccine candidates for bluetongue virus

BMC Biotechnology

Table 1 Primers and oligomers used for PCR of individual genes and the assembly PCR of fusion products

Gene/Oligomer	Size (bp)	Primer	Restriction sites (5′ / 3′)	5′ – 3′ sequence^a	Tm (°C)
Zera®	352	Zera®-FP^b,c Zera®-RP	AgeI NcoI	GCACCGGTATGAGGGTGTTGCTCGTT	62.7 58.3
Zera®	352	Zera®-FP^b,c Zera®-RP	AgeI NcoI	GCCCATGGCTGGCACGGGCTTGGAT	62.7 58.3
VP2ep	70	VP2ep-F VP2ep-R	MluI XmaI	GCACGCGTTTCCCAGAGCACCTTCGACACGATGTAACAAAT	76.7 79.8
VP2ep	70	VP2ep-F VP2ep-R	MluI XmaI	GCCCCGGGTCCACGCAGGCAGGGATACGGATTTGTTACATC	76.7 79.8
Linker			NcoI/MluI	CCATGGGAAGCGGCGGCGAAAACGCGT	75.9
		Linker-VP2ep-FP^b		GAAGCGGCGGCGAAAACGCGTTTCCCAGAGCACCTT	77.3
		Linker-Zera®-RP^b		TTTCGCCGCCGCTTCCCATGGTCTGGCACGGGCTTG	81.4
		VP2ep-RP^b,c	XmaI	GCCCCGGGTCCACGCAGGCAGGG	56.8
VP2	~2800	cTPVP2coF	MluI	GTGGACGCGTTAGGTGCATGGAAGAACTCGCTATCCCAA	56
		cVP2coR	XhoI	GCCTCGAGTCAAACGTTGAGGAGCTTAGTAAG	54
Sequencing primers		pEAQ-FP pEAQ-RP		GACGAACTTGGAGAAAGATTGTTAAGC	61.2 62.3
Sequencing primers		pEAQ-FP pEAQ-RP		AACCAGAGTTAAAGGCCTCGAGC	61.2 62.3

ISSN: 1472-6750