Fig. 2
From: Development of plant-produced protein body vaccine candidates for bluetongue virus
Expression of Zera®-VP2ep and Zera®-VP2 in N. benthamiana. Western blots of crude leaf extracts from leaves infiltrated with Zera®-VP2ep (a) and Zera®-VP2 (b) constructs at infiltration OD600 values of 0.5 and 1.5, respectively and harvested at 2, 3 5 and 7 days post infiltration (dpi). Expressed protein was detected with α-VP2R polyclonal antibody. Samples from negative control plants (−) were infiltrated with infiltration medium only. Red arrows indicate the position of the appropriately-sized expressed proteins. Lanes M represent the molecular weight marker with sizes indicated in kDa. c N. benthamiana leaves infiltrated with different A. tumefaciens cell concentrations. Leaves expressing Zera®-VP2ep and Zera®-VP2 were photographed at 3 and 7 dpi, respectively