Table 1 Primers utilized in this study
Primer name | Sequence |
|---|---|
GLuc-F | TTGGCGCGCCGCCACCATGGGAGTCAAA |
GLuc-R | GAGGCTGATTTTGCGTCTAGA |
T7 | TAATACGACTCACTATAGGG |
Seq-R | TTACGCCAAGTTATTTAGGTGACA |
H3-F | AAGGAGCTCGAGCCACCATGGCTCGT |
H3-R | GGTTACCTTAAGCACGTTCTCCA |
SGLuc8990–MF | CCAAGATGAAGAAGTGGCTCCCAGGACGCTGCC |
SGLuc8990–MR | GGCAGCGTCCTGGGAGCCACTTCTTCATCTTGG |
GLuc-NS-NheI-R | CGGCGCTAGCGTCACCACCGGCCCCCTT |
AscI-Kzk-GLuc-F | TTGGCGCGCCGCCACCATGGGAGTCAAA |
2A-XmaI-R | TATACCCGGGTCCAGGGTTGGACTC |
GLuc-R-NotI | GCGGCCGCTTAGTCACCACCGGCCCC |
GLuc8990–MF | CCAAGATGAAGAAGTTCATCCCAGGACGCTGCC |
GLuc8990–MR | GGCAGCGTCCTGGGATGAACTTCTTCATCTTGG |
No Met GLuc-MF | TCCAACCCTGGGCCCGGAGTCAAAGTTCTG |
No Met GLuc-MR | CAGAACTTTGACTCCGGGCCCAGGGTTGGA |
NotI-3CLeb89-F | CAGCGGCCGCATGAGTGGTGCCCCACCG |
3CLeb89-EcoRI-R | GAATTCCTACTCGTGGTGTGGTT |
3CLeb89-ns-EcoRI-R | GAATTCCTCGTGGTGTGGTTC |