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Table 1 Summary of the advantages and disadvantages of the four delivery methods evaluated

From: AAV vector distribution in the mouse respiratory tract following four different methods of administration

Technique Anatomic Target Advantages Disadvantages
Intranasal - Nasal epithelium
- Tracheal epithelium
- Proximal aspect of lung lobes
- Relatively quick
- Little skill/training required
- Can be performed by one person
- Inexpensive
- Best option if wanting to target the upper respiratory tract
- Can administer multiple doses
- Frequent loss of vector due to swallowing
- Inefficient delivery to the
lower respiratory tract
Modified intranasal - Nasal epithelium
- Tracheal epithelium
- Proximal to middle aspect of lung lobes
- Relatively quick
- Little skill/training required
- Inexpensive
- Least variable delivery method
- Can administer multiple doses
- Requires two people
- Does not reach the distal aspect of lung lobes
Intubation - Nasal epithelium
- Tracheal epithelium
- Proximal, middle and distal aspect of lung lobes
- Method that most
consistently reaches the distal aspect of the lung lobes
- Can be performed by one person
- Technically challenging
- Requires more equipment than the other methods
- Variable
- Time consuming
- Possibility of losing
vector if esophagus is penetrated
- Excessive swelling prevents repeated attempts at intubation
Intratracheal - Nasal epithelium
- Tracheal epithelium
- Proximal to middle
aspect of lung lobes
- Possible to target distal aspect of lung lobes
- Primarily targets the lower
respiratory tract
- Limited delivery to the nasal epithelium
- Can be performed by one person, but is easier with two people
- Technically challenging
- Requires surgical skills
- Variable
- Invasive
- Requires analgesic
- Re-administration not advised if done more than 5 days after initial administration
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