Fig. 6

Histological analysis of alkaline phosphatase staining in the lungs of mice 21 days after delivering 1x10¹¹ vg of an AAV6 vector expressing hPLAP using four different methods of administration. Viral vectors were administered by the intranasal (a and b), modified-intranasal (c and d), intubation (e and f), or intratracheal injection (g and h) method. Tissue sections of one representative lung lobe stained for hPLAP expression and counterstained with nuclear fast red are shown at 4x (A, C, E, F; scale bar = 200 μM) and 10x (B, D, F, H; scale bar = 100 μM) magnification