Fig. 2

Glucose caused deactivation of PFK1 activities in the sfPFKM transformants. In glycerol/ethanol medium pre-grown cells Pfk1 specific activities were determined in the homogenates of the wild-type HD56-5A strain, nPFKM and sfPFKM transformants. No activity was observed in the host pfk null HD114-8D strain with the empty plasmid (a). However after the transfer of glycerol/ethanol pre-grown cells to the glucose medium Pfk1 activities were rapidly lost in the strains with sfPFKM genes (b). Data are present as means ± standard deviation