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Fig. 5 | BMC Biotechnology

Fig. 5

From: Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

Fig. 5

Reduced expression of polySia in recombinant CHO cells mediated by anti-ST8SiaII and anti-ST8SiaIV IBs. a Immunoblot analysis of expressed NCAM and polySia of NCAM in CHO-2A10 + 500-cells expressing ST8SiaII transfected with ST8SiaII-IB. Negative control: cells non transfected or transfected with pCMV/myc/ER. Positive control: cell lysat treated with endosialidase. b Immunoblot analysis of expressed NCAM and polySia of NCAM in CHO-2A10 + 418-cells expressing ST8SiaIV transfected with ST8SiaIV IB. The experiment was done 3 times. Shown is a characteristic example. Sample volume: 10 μl of 100 μl cell lysat from 106 cells grown for 48 h in a 6-well microtiter plate

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BMC Biotechnology

ISSN: 1472-6750

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