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Fig. 3 | BMC Biotechnology

Fig. 3

From: Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

Fig. 3

Intracellular Binding of anti-ST8SiaII-IB and anti-ST8SiaIV-IB to their antigens. a control, immunoprecipitation of FLAG-HA tagged ST8SiaII and FLAG-HA tagged ST8SiaIV transiently transfected in 106 HEK293 cells for 48 h in a 6-well microtitre plate. After lysis in 100 μl lysisbuffer and immunoprecipitation the different glycosylated forms were analyzed by immunoblotting. Negative control: transfection with empty vector pcDNA3-FLAG-HA. b Co-IP of HEK 293 cells cotransfected with FLAG-HA tagged ST8SiaII and ST8SiaII-IB or FLAG-HA tagged ST8SiaIV and ST8SiaIV-IB. Negative control: Co-IP of HEK293 cells cotransfected with pcDNA3-FLAG-HA and anti-ST8SiaII-IB expressionplasmid. ImB: Immunoblot; ImP: Immunoprecipitation. Sample volume is 12 μl from total 25 μl immunoprecipitat. The experiment was done 2 times, shown is a representative example

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BMC Biotechnology

ISSN: 1472-6750

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