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Fig. 2 | BMC Biotechnology

Fig. 2

From: Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

Fig. 2

Binding of anti-ST8SiaII-IB and anti-ST8SiaIV-IB to their antigens in ELISA. a 50 ng purified ST8SiaII and ST8SiaIV in 50 μl 0.2 M sodium phosphate puffer was immobilized on MaxiSorb™ polystyrene assay plates (Nunc) as indicated. Serial dilutions of purified original anti-ST8SiaII and anti-ST8SiaIV mAbs 3167 and 3175, respectively, were applied in 100 μl PBS. Negative control: ST8SiaIV incubated with anti-myc antibody. b Serial dilutions of 100 μl cell lysates of 106 HEK293 cells transiently transfected with anti-ST8SiaII-IB expression plasmid or anti-ST8SiaIV-IB expression plasmid for 48 h in a 6 well microtiter plate were incubated in different serial dilutions in 100 μl PBS with immobilized purified ST8SiaII or ST8SiaIV. Negative controls: Cell lysates transfected with pCMV/myc/ER. Result of 3 independent experiments. Bars demonstrate standard deviation calculated from the mean values

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