Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

Table 5 Synergy between the commercial chitinase CtnSg and rHJ5Nag for the degradation of colloidal chitin

Order of enzyme addition and reaction time				Reducing sugars (μmol)	Degree of synergy^a
First enzyme	Time (min)	Second enzyme	Time (min)	Reducing sugars (μmol)	Degree of synergy^a
CtnSg	120	No	0	0.28	1.00
rHJ5Nag	120	No	0	ND^b	-
CtnSg & rHJ5Nag	120	No	0	0.57	2.02
CtnSg	30	rHJ5Nag	90	0.55	1.98
CtnSg	60	rHJ5Nag	60	0.48	1.72
CtnSg	90	rHJ5Nag	30	0.36	1.29

^aThe degree of synergy was calculated using the following equations:
\( D S=\frac{R{S}_{rHJ5 Nag CtnSg}}{R{S}_{rHJ5 Nag}+ R{S}_{CtnSg}} \)
where DS is the degree of synergy, RS _rHJ5NagCtnSg is the amount of reducing sugars released from simultaneous or sequential enzyme reactions, and RS _rHJ5Nag and RS _CtnSg are the amounts of reducing sugars released from individual enzyme reactions
^bNot detected

ISSN: 1472-6750