Skip to main content

Table 3 Real-time RT-PCR analysis

From: Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

Sample

Gene expression log2 fold change a [log2 (2-ΔΔCT (Exp-Control))]

Experimental/Control

thil

crt-bcd-etfB-etfA-hbd

adhe

ompC-tmt

BUT2-DE/BUT1-DE

-7.79 ± 0.02

-8.64 ± 0.08

-7.72 ± 0.06

-1.40 ± 0.02

BUT3-DE/BUT1-DE

-3.09 ± 0.12

-4.14 ± 0.69

-0.76 ± 0.10

2.84 ± 0.09

BUT3-DE/BUT2-DE

4.70 ± 0.11

4.50 ± 0.68

6.96 ± 0.16

4.24 ± 0.08

  1. a The values represent ratio of gene expression log2 fold change and are means ± standard deviations from three independent experiments
  2. (Gene expression log2 fold change: >0, ie. Up regulation; ≒0, ie. No change; < 0, ie. Down regulation)