Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

Fig. 1

Structure of the plasmids pBUT, pBUT-rO-tMT and pBUT-T7-rO-tMT. Three recombinant plasmids were derived from the expression vector pGETS118 [29, 74], which contained the Pr promoter. To assemble the butanol synthesis pathway, target gene(s) thil (1.2 kb), crt-bcd-etfB-etfA-hbd cluster (4.8 kb), adhe (2.8 kb) and rO-tMT (1.3 kb) or T7-rO-tMT (1.4 kb) were inserted under the Pr promoter, resulting in pBUT (a), pBUT-rO-tMT (b), and pBUT-T7-rO-tMT (c), respectively. All the plasmids include two antibiotic resistances (Tcr : tetracycline resistance; CATr : chloramphenicol resistance)

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us