Table 6 Validation of microarray results using quantitative real-time PCR
From: Abnormal gene expression in regular and aggregated somatic cell nuclear transfer placentas
Gene symbol | Accession No. | Microarray folder Δ, SCNT/Con | Expression | qRT-PCR folder Δ, SCNT/Con |
|---|---|---|---|---|
Plac1 | NM_019538.3 | 2.73** | ↑NT | 6.54 |
Slc38a4 | NM_027052.3 | 2.32** | ↑NT | 4.04* |
Rprml | NM_001033212.1 | 3.25** | ↑NT | 4.51 |
Pla2g4f | NM_001024145.1 | 4.10** | ↑NT | 3.13* |
Pla2g4d | NM_001024137.1 | 3.70** | ↑NT | 9.04 |
Hsd17b7 | NM_010476.3 | 3.24** | ↑NT | 7.66 |
Hmox1 | NM_010442.1 | 2.73** | ↑NT | 4.30 |
Chac1 | NM_026929.3 | −2.27* | ↓NT | −8.41 |
Car2 | NM_009801.3 | 3.95** | ↑NT | 6.11 |
Slpi | NM_011414.2 | −4.56** | ↓NT | −4.06 |
Nrn1l | NM_175024.3 | −2.49** | ↓NT | −3.05** |
H19 | NR_001592.1 | 1.10 | 1.19 |