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Table 3 Propionate production of engineered strains in NBS (20 g/L glucose) media with 250 mL mini bioreactors

From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production

  Titer (g/L) Yield (Propionate/Glucose)
  Succinate Acetate Propionate Ethanol (g/g) (mol/mol)
T110 (plac-Sbm) 15.49 ± 0.05 2.36 ± 0.12 0.49 ± 0.21 0.75 ± 0.08 0.02 ± 0.009 0.05 ± 0.02
T110 (pbba-Sbm) 9.27 ± 0.02 0.49 ± 0.14 4.75 ± 0.5 0 0.19 ± 0.02 0.48 ± 0.05
T110 (pbba-Sbm, plac-ABb) 6.10 ± 0.21 0.38 ± 0.08 3.05 ± 0.12 0 0.19 ± 0.005 0.47 ± 0.01
T110 (pbba-Sbm, pbba-ABb) 8.48 ± 0.20 0.65 ± 0.11 4.95 ± 0.08 0 0.20 ± 0.003 0.49 ± 0.007
  1. Strains were cultivated aerobically in the dark in 250 mL mini bioreactors in NBS (20 g/L glucose) media at 37 °C, until the cultures reached at an OD600 of 0.5. Then IPTG with appropriate concentration was added and cultivated anaerobically in the dark for 72 h at 30 °C, for methylmalonyl-CoA mutase from M. extorquens AM1 was active at 30 °C. All experiments were performed in triplicate