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Table 3 Propionate production of engineered strains in NBS (20 g/L glucose) media with 250 mL mini bioreactors

From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production

 

Titer (g/L)

Yield (Propionate/Glucose)

 

Succinate

Acetate

Propionate

Ethanol

(g/g)

(mol/mol)

T110 (plac-Sbm)

15.49 ± 0.05

2.36 ± 0.12

0.49 ± 0.21

0.75 ± 0.08

0.02 ± 0.009

0.05 ± 0.02

T110 (pbba-Sbm)

9.27 ± 0.02

0.49 ± 0.14

4.75 ± 0.5

0

0.19 ± 0.02

0.48 ± 0.05

T110 (pbba-Sbm, plac-ABb)

6.10 ± 0.21

0.38 ± 0.08

3.05 ± 0.12

0

0.19 ± 0.005

0.47 ± 0.01

T110 (pbba-Sbm, pbba-ABb)

8.48 ± 0.20

0.65 ± 0.11

4.95 ± 0.08

0

0.20 ± 0.003

0.49 ± 0.007

  1. Strains were cultivated aerobically in the dark in 250 mL mini bioreactors in NBS (20 g/L glucose) media at 37 °C, until the cultures reached at an OD600 of 0.5. Then IPTG with appropriate concentration was added and cultivated anaerobically in the dark for 72 h at 30 °C, for methylmalonyl-CoA mutase from M. extorquens AM1 was active at 30 °C. All experiments were performed in triplicate
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BMC Biotechnology

ISSN: 1472-6750

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