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Table 2 Fermentation profiles of E. coli strains engineered with novel propionate fermentation pathway

From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production

 

Titers (g/L)

Yield (Propionate/Glucose)

 

Succinate

Lactate

Acetate

Propionate

Ethanol

(mol/mol)

MG1655

1.00 ± 0.11

7.12 ± 0.21

2.85 ± 0.06

0.08 ± 0.02

0.67 ± 0.14

0.013 ± 0.002

MG1655 (plac-Sbm)

0.28 ± 0.07

1.91 ± 0.34

3.12 ± 0.74

0.24 ± 0.02

0.61 ± 0.25

0.039 ± 0.005

T110

10.99 ± 0.18

0

2.62 ± 0.19

0.01 ± 0.01

0

0

T110 (plac-Sbm)

9.98 ± 0.12

0

1.56 ± 0.09

0.43 ± 0.07

0

0.068 ± 0.002

T110ΔducBC

2.85 ± 0.25

0

1.48 ± 0.15

0.008 ± 0.005

0

0

T110ΔducBC (plac-Sbm)

1.45 ± 0.31

0

1.09 ± 0.27

0.13 ± 0.06

0

0.021 ± 0.005

  1. Strains in glass screw-cap tubes were cultivated aerobically in the dark at 37 °C, until the cultures reached an OD600 of 0.5. Then culture was added with 1 mM IPTG and cultivated anaerobically in the dark for 72 h. All experiments were performed in triplicate
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BMC Biotechnology

ISSN: 1472-6750

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