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Table 2 Fermentation profiles of E. coli strains engineered with novel propionate fermentation pathway

From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production

  Titers (g/L) Yield (Propionate/Glucose)
  Succinate Lactate Acetate Propionate Ethanol (mol/mol)
MG1655 1.00 ± 0.11 7.12 ± 0.21 2.85 ± 0.06 0.08 ± 0.02 0.67 ± 0.14 0.013 ± 0.002
MG1655 (plac-Sbm) 0.28 ± 0.07 1.91 ± 0.34 3.12 ± 0.74 0.24 ± 0.02 0.61 ± 0.25 0.039 ± 0.005
T110 10.99 ± 0.18 0 2.62 ± 0.19 0.01 ± 0.01 0 0
T110 (plac-Sbm) 9.98 ± 0.12 0 1.56 ± 0.09 0.43 ± 0.07 0 0.068 ± 0.002
T110ΔducBC 2.85 ± 0.25 0 1.48 ± 0.15 0.008 ± 0.005 0 0
T110ΔducBC (plac-Sbm) 1.45 ± 0.31 0 1.09 ± 0.27 0.13 ± 0.06 0 0.021 ± 0.005
  1. Strains in glass screw-cap tubes were cultivated aerobically in the dark at 37 °C, until the cultures reached an OD600 of 0.5. Then culture was added with 1 mM IPTG and cultivated anaerobically in the dark for 72 h. All experiments were performed in triplicate