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Table 1 Strains and plasmids used in this study

From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production

Name Description, relevant genotype Source
Strains
 MG1655 Wild type Lab collection
 T110 ATCC 8739ΔldhA ΔpflB ΔptsI Ppck*-galP-pcka [30]
 T110ΔducBC T110 ΔducB ΔducC [31]
Plasmids
 pET28a   Lab collection
 pACYC184   Lab collection
 pEC-XK99E   Lab collection
 plac-Sbm Derived from pET28a,Plai:: sbm-ygfD-ygfG This study
 ptac-Sbm Derived from pET28a,Ptac:: sbm-ygfD-ygfG This study
 pbba-Sbm Derived from pET28a,Pbba:: sbm-ygfD-ygfG This study
 plac-ABb Derived from pACYC184,Plac:: mcmA-mcmB-meab This study
 pbba-ABb Derived from pACYC184,Pbba:: mcmA-mcmB-meab This study
  1. aPpck* is a mutated form of the pck promoter (G to A at position 64 relative to the ATG start codon) [30]