Fig. 3From: Construction of a novel anaerobic pathway in Escherichia coli for propionate productionThe effects of expression of M. extorquens AM1 Methylmalonyl CoA mutase on propionate production. Strains were cultivated aerobically in glass screw-cap tubes in dark at 37 °C, until the cultures reached at an OD600 of 0.5. Then IPTG with appropriate concentration was added and cells were cultivated anaerobically in the dark for 72 h at 30 °C. All experiments were performed in triplicateBack to article page