Skip to main content

Table 1 Cell lines, secreting FVIII

From: Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

Cell line name Steps of selection and target gene amplification by MTX FVIII concentration, IU/ml Specific productivity, μIU/cell/day
3-B8 50 nM 5.4 ± 0.4a -
3-B8 1 K 50 nM → 500 nM → 1 μM 20.1a -
3-B8 2 K 50 nM → 500 nM → 2 μM 18.8a -
3-B8 1 K 2 K 50 nM → 500 nM → 1 μM → 2 μM 17.6 -
3-B8 1 K 4 K 50 nM → 500 nM → 1 μM → 4 μM 18.6 -
13B4F 50 nM → 500 nM → 1 μM → 2 μM → cloning 39.0 ± 5.5 11.8
11A4H 50 nM → 500 nM → 1 μM → 2 μM → cloning 39.4 ± 3.4 14.2
21A7B 50 nM → 500 nM → 1 μM → 2 μM → cloning 34.4 ± 0.8 11.8
  1. a- FVIII concentration determined by ELISA