Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

Table 1 Cell lines, secreting FVIII

Cell line name	Steps of selection and target gene amplification by MTX	FVIII concentration, IU/ml	Specific productivity, μIU/cell/day
3-B8	50 nM	5.4 ± 0.4^a	-
3-B8 1 K	50 nM → 500 nM → 1 μM	20.1^a	-
3-B8 2 K	50 nM → 500 nM → 2 μM	18.8^a	-
3-B8 1 K 2 K	50 nM → 500 nM → 1 μM → 2 μM	17.6	-
3-B8 1 K 4 K	50 nM → 500 nM → 1 μM → 4 μM	18.6	-
13B4F	50 nM → 500 nM → 1 μM → 2 μM → cloning	39.0 ± 5.5	11.8
11A4H	50 nM → 500 nM → 1 μM → 2 μM → cloning	39.4 ± 3.4	14.2
21A7B	50 nM → 500 nM → 1 μM → 2 μM → cloning	34.4 ± 0.8	11.8

ISSN: 1472-6750