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Fig. 7 | BMC Biotechnology

Fig. 7

From: Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

Fig. 7

Analysis of the purified FVIII-BDD. Panel a - FVIII purification steps by SDS-PAGE. Panel b – Western blotting of purified FVIII. Panel c - tryptic peptides, identified by MALDI-TOF mass spectrometry. M – marker; MMC wash – wash fraction stage 1; SP el – elution fraction stage 2; VIII ff – flow-through fraction stage 3; VIII el – elution fraction stage 3. HC – heavy chain, LC – light chain. SDS-PAGE in reducing conditions, molecular weights are shown in kDa. Images were contrast enhanced to add visibility. Identified peptides are marked in yellow

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BMC Biotechnology

ISSN: 1472-6750

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