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Fig. 4 | BMC Biotechnology

Fig. 4

From: Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

Fig. 4

qPCR analysis of FVIII mRNA and expression levels of various housekeeping genes for clonal FVIII-secreting cell lines. Panel a – levels of FVIII mRNA relative to β-actin mRNA. Panel b - expression levels of genes involved in protein synthesis and processing compared to the parent cell line CHO DG-44. Sample “CMV-F8” on the panel a – clonal cell line DG-BDDFVIII-18, described in [18]. Data on the panel b normalized to β-actin. EiF1a1- eukaryotic translation initiation factor 1a, EiF3- eukaryotic initiation factor 3, PPIB- peptidyl-propyl isomerase B, BIP –immunoglobulin-binding protein (BiP, Grp78); OSTC- oligosaccharyltransferase complex subunit; St3gal – ST3 beta-galactoside alpha-2,3-sialyltransferase 3; B4gal - beta-1,4-galactosyltransferase 1. FIXp – control line producing factor IX. One representative experiment from 3 is shown. Error bars represent standard deviation, n = 3-4

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BMC Biotechnology

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