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Fig. 2 | BMC Biotechnology

Fig. 2

From: Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

Fig. 2

qPCR analysis of the expression cassette copy numbers per haploid genome. Panel a - average copy number increase during MTX-driven amplification in oligoclonal cell lines. Panel b - expression cassette copy number per haploid genome for clonal lines 13B4F, 11A4H, 21A7B. Panels c, d - copy numbers for the control sequence (PPIB), supposedly unique to the CHO genome. ID- primers toward IRES-DHFR region, F8B - primers toward FVIII-BDD ORF. Positions of the amplicons on the plasmid are depicted on Fig. 1a. One representative experiment from 3 is shown. Error bars represent standard deviation, n = 3

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