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Table 2 Kinetic parameters of WT 1593, WT sll0208 and some variants

From: Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

   K m (mM) k cat (min−1) k cat /K m (min−1mM−1)
1593 WT [17 0.30 ± 0.02 0.48 ± 0.01 1.6 ± 0.2
L146T 0.34 ± 0.08 0.94 ± 0.08 2.76 ± 0.3
F150Y 0.20 ± 0.04 0.75 ± 0.04 3.75 ± 0.4
Q49H/F150Y 0.33 ± 0.05 0.87 ± 0.04 2.64 ± 0.2
Q49H/N123H/F150Y 0.35 ± 0.02 1.04 ± 0.02 2.97 ± 0.3
W178R 0.34 ± 0.08 1.81 ± 0.15 5.32 ± 0.4
sll0208 WT 0.35 ± 0.07 0.59 ± 0.04 1.69 ± 0.2
L148R 0.32 ± 0.08 0.91 ± 0.08 2.84 ± 0.3
D49H/N123H 0.38 ± 0.08 0.83 ± 0.08 2.18 ± 0.2
  1. The kinetic parameters against n-heptanal were determined