Table 1 All primers used in this study

CNA-qRT-F and CNA-qRT-R

Partial fragment of CNA gene was 190 bp

AACGGTGCGGAGGGTAT

GCTGTGCCAGTCTTGTCG

18S-F and 18S-R

Partial fragment of 18S rDNA gene was 198 bp

ATGCTGAAAAGCCCCGACT

ATTCCCCGTTACCCGTTG

CaM-F and CaM-R

the fragment of CaM gene was 450 bp

5′-GAATTCATGGCGGAGCAGCTGACCAAG-3′(EcoRI site underlined)

5′-CTCGAGTTACTTGGCCATCATCATG GTAAC-3′ (XhoI site underlined)

CNA-F and CNA-R

the fragment of CNA gene was 1917 bp

5′-GAATTCATGACCTC TCCGGCGAC-3′

(EcoRI site underlined)

5′-GCGGCCGCTTAGGCAATAGAGTTC TC-3′ (Not I site underlined)

CNAa-F and CNAa-R

1-1284 bp fragment of CNA, excluding calmodulin binding domain

5′-GAATTCATGACCTCTCCGGCGAC-3′

(EcoRI site underlined)

5′-GCGGCCGCCCTC TCCGCCGAGATGTC -3′ (Not I site underlined)

CNAb-F and CNAb-R

1-1362 bp fragment of CNA, including calmodulin binding domain

5′-GAATTCATGACCTCTCCGGCGAC-3′ (EcoRI site underlined)

5′-GCGGCCGCCGAGAACACTCGCGACAT-3′ (Not I site underlined)

mut-∆CNA1-F and mut-∆CNA1-R

Replace Ile439 with Ala439

GGAGAGGAGACAGGTTGCCAAGAACAAGATTCTC

GAGAATCTTGTTCTTGGCAACCTGTCTCCTCTCC

mut-∆CNA2-F and mut-∆CNA2-R

Replace Ile443 with Ala443

GGTTATCAAGAACAAGGCTCTCGCTGTTGGTCGC

GCGACCAACAGCGAGAGCCTTGTTCTTGATAACC

mut-∆CNA3-F and mut-∆CNA3-R

Replace Val446 with Ala446

AACAAGATTCTCGCTGCTGGTCGCATGTCGCGA

TCGCGACATGCGACCAGCAGCGAGA ATCTTGTT

mut-∆CNA4-F and mut-∆CNA4-R

Replace Val452 with Ala452

GGTCGCATGTCGCGAGCGTTCTCGTTGCTTCGT

ACGAAGCAACGAGAACGCTCGCGACATGCGACC

mut-∆CNA5-F and mut-∆CNA5-R

Replace Phe453 with Ala453

CGCATGTCGCGAGTGGCCTCGTTGC TTCGTGAG

CTCACGAAGCAACGAGGCCACTCGC GACATGCG