Fig. 4

Analysis of the interaction between the recombinant proteins and CaM by Far-Western blot. a Far-Western blot analysis of the binding of CNA, ∆CNAb, ∆CNAa with CaM. Lane 1, protein standards; Proteins samples were 40 μg. b Analysis of the relative binding capacities of the truncated proteins with CaM. c Far-Western blot analysis of the binding of CNA, CNA1, CNA2, CNA3, CNA4, CNA5 with CaM. Lane 1, protein standards. Proteins samples were 20 μg. d Analysis of the relative binding capacities of the site-directed mutagenesis proteins with CaM. Relative activity analysis was performed by image J. The binding capacity of CNA with CaM was designated as 1