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Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

BMC Biotechnology volume 17, Article number: 29 (2017) Cite this article

Abstract

Background

Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have been used for transgenic breeding and gene function study, a quick, easy and large-scale transformation platform has been in urgent need for gene functional characterization, especially after the cassava full genome was sequenced.

Methods

Fully expanded leaves from in vitro plantlets of Manihot esculenta were used to optimize the concentrations of cellulase R-10 and macerozyme R-10 for obtaining protoplasts with the highest yield and viability. Then, the optimum conditions (PEG4000 concentration and transfection time) were determined for cassava protoplast transient gene expression. In addition, the reliability of the established protocol was confirmed for subcellular protein localization.

Results

In this work we optimized the main influencing factors and developed an efficient mesophyll protoplast isolation and PEG-mediated transient gene expression in cassava. The suitable enzyme digestion system was established with the combination of 1.6% cellulase R-10 and 0.8% macerozyme R-10 for 16 h of digestion in the dark at 25 °C, resulting in the high yield (4.4 × 107 protoplasts/g FW) and vitality (92.6%) of mesophyll protoplasts. The maximum transfection efficiency (70.8%) was obtained with the incubation of the protoplasts/vector DNA mixture with 25% PEG4000 for 10 min. We validated the applicability of the system for studying the subcellular localization of MeSTP7 (an H+/monosaccharide cotransporter) with our transient expression protocol and a heterologous Arabidopsis transient gene expression system.

Conclusion

We optimized the main influencing factors and developed an efficient mesophyll protoplast isolation and transient gene expression in cassava, which will facilitate large-scale characterization of genes and pathways in cassava.

Background

Transient gene expression in plant protoplasts is a powerful technique for studying subcellular localization of proteins, gene and promoter activities, protein-protein interactions, and signal transduction [1–7]. Compared with the stable gene expression in transgenic plants, transient gene expression represents a fast, convenient and efficient alternative system with higher expression levels [8–10]. Moreover, the transient gene expression assay enable the high-throughput analysis of gene functions while the stable gene expression is relatively expensive and time-consuming thus limiting the utilization of this technique for large-scale analyses of plant genes [8–10]. Although different transfection techniques such as polyethylene glycol (PEG)-mediated, electroporation and microinjection have been developed to deliver recombinant DNA plasmids into protoplasts, utilization of the PEG-mediated approach has high transformation efficiency, so it is widely applied in molecular and cellular studies for both model plants and non-model plants [4, 7, 8, 11–20].

Cassava (Manihot esculenta Crantz) is a perennial and woody shrub of the Euphorbiaceae cultivated in tropical and subtropical regions for its starchy storage roots [21, 22]. These roots have been used as the sources for dietary carbohydrate, starch processing and potential biofuel production. Since that cassava is a staple crop for approximately 800 million people in developing regions of the tropics [23], many groups have been intensively performing research on cassava molecular breeding which relies on the identification of agronomically important genes and pathways. Recently, the cassava sequencing data set has been publicly released [24] and the determination of gene function has been a major goal of cassava molecular biology in genomic and post-genomic era, thus it will necessitate efficient high-throughput transient gene expression for gene function analysis.

Stable gene transfer can be developed for gene function studies at whole plant level. Much progress has been made in cassava genetic transformation of both Agrobacterium tumefaciens-mediated and biolistic-mediated system [23, 25–27]. However, the transformation efficiency is relatively low and genotypic dependent [28], and the process requires well-trained tissue culture specialists, is lengthy and difficult to repeat [29, 30]. Hence, the development of an efficient transient gene expression system that could be exploited to characterize gene functions and investigate molecular processes in cassava is required.

Due to the fact that cassava is recalcitrant for plant regeneration from protoplasts, only two reports about the protoplast culture and regeneration of cassava have been published. Shahin and Shephard reported shoot regeneration from the isolated mesophyll protoplasts of cassava [31] and Sofiari et al. reported the plant regeneration from protoplasts isolated from friable embryogenic callus of cassava [32]. Anthony et al. developed a protocol for the culture of cassava leaf protoplasts but plant regeneration from protoplasts had not achieved [33]. To date, transient gene expression in cassava protoplasts has not been developed. Here we report an effective and reliable mesophyll protoplast isolation and PEG-mediated transient gene expression in cassava. The protocol has been successfully used for subcellular localization of a sugar transporter, MeSTP7 protein.

Methods

Materials

In vitro plantlets of Manihot esculenta cv. South China 8 (SC8) were used in this study. Cultures were kept on 1/2MS (Murashige and Skoog) medium (supplemented with 2% sucrose and 0.8% agarose, pH5.8) at 28 °C, under lighting with a cycle of 12 h/8 h (light/darkness) for 6-10 weeks to obtain fully expanded leaves.

Protoplast isolation

Protoplast isolation was conducted using the protocols described by Yoo et al. [3] and Anthony et al. [33] with slight modification. The fully expanded green leaves were cut into about 0.5-1.0 mm strips with sharp razors. The strips of 0.3 ± 0.03 g (for each treatment) were transferred quickly into 10 mL of enzyme solution (0.1% BSA, 9% mannitol, 20 mM KCl, 10 mM CaCl2, 20 mM MES, pH 5.8) with different concentrations of cellulase R-10 (0.8%, 1.6% or 2.4%) (Yakult, Japan) and macerozyme R-10 (0.4%, 0.8% or 1.2%) (Yakult, Japan) for cell wall hydrolysis by shaking at 45 rpm for 16 h in the dark (25 °C) for plasmolysis.

The digested tissues were filtered through a 0.75-mm nylon sieve, collected by centrifugation (80 g for 3 min) and suspended in 20 mL pre-cooled W5 solution (154 mM NaCl, 125 mM CaCl2, 5 mM KCl and 2 mM MES, pH 5.8). The protoplast pellet was purified twice in W5 solution by repeated resuspension and centrifugation (80 g for 3 min).

The resulting protoplasts were resuspended in 2 mL W5 solution, placed on the ice for 30 min and counted under a microscope equipped with a hemocytometer. The viability of protoplasts was measured with 0.2% fluorescein diacetate (FDA) staining and determined as follows: protoplast viability (%) = (fluorescent protoplast number in view/protoplast total number in view) × 100%.

Cassava mesophyll protoplast transformation

The protoplasts were collected by centrifugation (80 g for 2 min) and resuspended in MMg solution (9% mannitol, 15 mM MgCl2, 4 mM MES) to a density of 1.0 × 107 protoplasts/ml. 15 μg of pA7-GFP plasmid [34] were mixed gently with 200 μl protoplasts. Then different concentrations of freshly prepared PEG4000 solutions (9% mannitol, 100 mM CaCl2) were immediately added and mixed by gentle inversion to obtain a 15%, 20%, 25% or 30% final PEG4000 concentration. The mixture was incubated at room temperature for 5, 10, 15 and 20 min, respectively. After incubation, the transfection mixture was gently diluted with three volumes of W5 solution.

The transfected protoplasts were centrifuged (80 g, 2 min) twice, then resuspended with 300-500 μl WI solution (4 mM MES, 9% mannitol, 20 mM KCl, pH 5.8) and incubated overnight at room temperature in dark for the induction of protein expression. Transformation efficiency was detected under fluorescence microscopy and the expression of GFP tag was observed by confocal laser scanning microscope. Transformation efficiency was calculated as follows: transformation efficiency (%) = (bright green fluorescent protoplast number in view/total protoplast number in view).

Plasmid construction and subcellular localization of MeSTP7 protein

Total RNA was extracted from cassava leaves with RNAplant Plus (Tiangen biotech, Beijing, China) and cDNA was synthesized using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) following the manufacturer’s instructions.

The specific primers with Xho I and Spe I restriction enzyme sites (bold) introduced to their ends respectively, STP7-F (5'-CCGCTCGAGATGCCTGCAGGAGGTTT-3') and STP7-R (5'-GGACTAGTGAAAACTGGGTAACAGGATCAA-3'), were designed according to the conserved region of MeSTP7 (GenBank accession number: Manes.03G180400). The gene of interest was amplified using Phusion DNA Polymerase under the following conditions: 98 °C for 30 s followed by 35 cycles of amplification (98 °C for 10 s, 57 °C for 30 s, 72 °C for 45 s) and a 10-min final extension at 72 °C.

The PCR products were confirmed by sequencing and inserted between the 35S promoter and the GFP of the expression vector pA7-GFP, designated as pA7-MeSTP7-GFP (Fig. 1). The plasmid construct was propagated in competent Escherichia coli Trans-T1 (TransGen Biotech, Beijing, China). Subcellular localizations of MeSTP7 protein in cassava and Arabidopsis mesophyll protoplasts were carried out according to our transformation procedure described above and the method established by Yoo et al [3], respectively.

Fig. 1
figure 1

Schematic representation of the pA7-MeSTP7-GFP construct driven by the constitutive CaMV 35S promoter and terminated by nopaline synthase terminator (NOS)

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Results

Isolation of protoplasts from cassava leaves

Different enzyme combinations significantly influenced the yield and viability of cassava protoplasts. Protoplast yield increased with increasing concentrations of cellulase R-10 and macerozyme R-10, but protoplast viability decreased with increasing enzyme concentrations. It was found that the highest production of protoplasts (5.1 × 107 protoplasts/ g FW) was obtained with 2.4% cellulose and 1.2% macrerozyme, with the lowest viability of protoplasts (83.8%). For obtaining the highest number viable protoplasts for the subsequent transfection experiments, the optimum combination of enzymes for cassava protoplast isolation was determined as 1.6% cellulase + 0.8% macerozyme. This combination gave the yield of 4.4 × 107 protoplasts/g FW and the viability of 92.6%. The enzyme solution turned green, indicating the release of mesophyll protoplasts. Leaf mesophyll protoplasts were uniformly spherical, and containing single intact nucleus and green chloroplasts. (Table 1, Fig. 2).

Table 1 Effects of enzyme combinations on the yield and viability of protoplasts isolated from leaves of cassava
Full size table
Fig. 2
figure 2

The viability assays of cassava mesophyll protoplasts using the optimized protocol, i.e. preparation of mesophyll protoplasts in an enzyme solution (1.6% cellulase + 0.8% macerozyme) incubated at 25 °C (45 rpm) for 16 h in darkness. a Bright field image of protoplasts; (b) Protoplasts stained with FDA; (c) Merged image of protoplasts (Scale bar = 100 μm).

Full size image

Establishment of cassava mesophyll protoplast expression system

In this study, the effects of PEG4000 concentration and transfection duration on cassava protoplast transformation efficiency was investigated with the transient expression vector pA7-GFP. The results showed that when the transfection time was 10 min, the transfection efficiency increased along the increasing concentration of PEG4000 and reached the maximum level (70.8%) at the concentration of 25% (Figs. 3 and 4a). When the concentration of PEG4000 was 25%, transfection efficiency increased with increases in the transfection time until it peaked at 10 min (Figs. 3 and 4b). Therefore, factors affecting transfection efficiency were optimized: 25% of PEG4000 concentration and 10 min of transfection time. In addition, stability of transient expression of GFP has been verified as GFP signal could be detected 8-30 h after transformation.

Fig. 3
figure 3

High-efficiency transformation of cassava mesophyll protoplasts with pA7-GFP plasmid. a Bright field image of protoplasts; (b) Image of GFP; (c) Image of GFP merged with chlorophyll autofluorescence (Scale bar = 100 μm)

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Fig. 4
figure 4

Effects of PEG4000 concentration (a) and transfection time (b) on cassava protoplast transformation efficiency. Values are expressed as mean ± SEM, the error bars represent at least five independent replicates. The different letters indicate significant differences (P ≤ 0.05) according to the Duncan test

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Subcellular localization of GFP-fused MeSTP1 protein in cassava mesophyll protoplasts

To test the feasibility of the protoplast transient expression system for the subcellular localization of protein in cassava cells, the transient expression of GFP-fused MeSTP7 protein in cassava mesophyll protoplasts was performed. Twelve-eighteen hours after transfection, brilliant green fluorescence distributed in intracellular compartments was observed. MeSTP7:GFP was specifically detected in cell membrane, but empty vector control (GFP) was distributed throughout the nucleus and cytoplasm (Fig. 5). Similar pattern of MeSTP7 subcellular localization in Arabidopsis mesophyll protoplasts validated the authenticity of the results described above (Fig. 6).

Fig. 5
figure 5

Subcellular localization of MeSTP7 in cassava mesophyll protoplasts. Transient expression of GFP, showing that the GFP is distributed throughout the nucleus and cytoplasm (a-d). The laser-scanning confocal microscopy images are the bright field image (a), fluorescence image (b), merged image (c) and autofluorescence image (d), respectively. The transient expression of GFP-fused MeSTP7 protein, showing that the MeSTP7-GFP fusion protein is likely localized to plasma membrane (e-h). The laser-scanning confocal microscopy images are the bright field image (e), fluorescence image (f), merged image (g) and chloroplast fluorescence (H), respectively. The bars = 5 μm.)

Full size image
Fig. 6
figure 6

Subcellular localization analysis of MeSTP7 in Arabidopsis mesophyll protoplasts. Transient expression of GFP, showing that the GFP is distributed throughout the nucleus and cytoplasm (a-d). The laser-scanning confocal microscopy images are the bright field image (a), fluorescence image (b), merged image (c) and chloroplast fluorescence image (d), respectively. The transient expression of GFP-fused MeSTP7 protein, showing that the MeSTP7-GFP fusion protein is likely localized to cytoplasm membrane (e-h). The laser-scanning confocal microscopy images are the bright field image (e), fluorescence image (f), merged image (g) and chloroplast fluorescence image (h), respectively. The bars = 10 μm

Full size image

Discussion

A reliable protoplast isolation with high yield and viability is prerequisite for the success of transient gene expression. Source tissue, the type and concentration of enzymes are critical factors affecting the release of protoplasts [3, 35, 36]. Leaves from field or greenhouse-grown plants usually have a thick cuticle and recalcitrant cell walls, which are likely to be the major limiting factors for protoplast isolation [12]. In cassava, Shahin et al. [31] used well expanded leaves which were collected from preconditioned plants (in growth chamber) and then incubated on a solution supplemented with naphthaleneacetic acid (NAA) and benzylaminopurine (BAP) as protoplast source. Sofiari et al. [32] isolated cassava protoplasts from friable embryogenic callus (FEC) and from suspensions derived from FEC. Anthony et al. [33] took juvenile leaves from axenic shoot cultures of cassava as protoplast source. For convenience sake, we chose the leaves from in vitro propagated cassava plants as the protoplast source.

For a given species and source tissue, suitable enzymolysis time and enzymolysis composition are vital for protoplast isolation. Anthony et al. [33] had established an improved protocol for cassava protoplast isolation with an enzyme mixture for 16 h. Referring to the method described by Anthony et al. [33], optimization of the enzymolysis composition was conducted in this study. The combinations of cellulase and macerozyme which have been commonly used for protoplast isolation, were evaluated to obtain protoplasts with the highest yield and viability. As the concentration of total enzyme in the mixture increased, protoplast yield seemed to increase but protoplast viability to decrease. High levels of cellulose R-10 and macrerozyme R-10 increased protoplast yield but decreased the viability of protoplasts, possibly due to the influence of enzymes on the integrity of membrane and the physiological activities of protoplasts [37, 38]. The highest yield (5.1 × 107 protoplasts/ g FW) and the lowest viability of protoplasts (83.8%) were recorded with the 2.4% cellulose and 1.2% macrerozyme, which was the most concentrated enzyme mixture in our study. This lowest viability of protoplasts may not be suitable for subsequent experiments. However, the lowest concentrations of enzymes in the isolation mixture gave a lower yield (1.8 × 107 protoplasts/g FW) but with a slightly higher viability of protoplasts (93.6%). The enzyme mixture containing 1.6% Cellulase R10 and 0.8% macerozyme R10 was the best mixture in terms of both yield (4.4 × 107 protoplasts/g FW) and viability (92.6%) of protoplasts. The maximum yield and viability of leaf mesophyll protoplasts achieved in our study were substantially higher than the results previously reported [28, 30]. The reason possibly attributed to the differences in tissue source used [31, 32], and/or the type and concentration of enzymes [32, 33]. In addition, the high yield (4.4 × 107 protoplasts/g FW) and vitality (92.6%) of cassava mesophyll protoplasts obtained in our study were comparable to the values reported for Populus (1 × 107 protoplasts/g FW, more than 90%) [13], Brachypodium distachyon (1.7 × 107 protoplasts/g FW, more than 90%) [19], cucumber (6-7 × 107 protoplasts/g FW, about 90%) [15]. The high yield and viability allowed the application of this system for the subsequent transfection experiment.

Although heterologous transient gene expression systems with Arabidopsis and tobacco protoplasts are commonly adopted for studying gene function in non-model plants such as cassava, these heterologous systems may display aberrant results due to a foreign genetic background. In this study, we established an efficient cassava protoplast transfection system by optimizing PEG4000 concentration and transfection time. This protocol will be an extraordinarily valuable tool for cassava gene functional study. In addition, transfection efficiency over 50% is generally required for obtaining reliable and reproducible results with the protoplast system [3]. The highest transfection efficiency (70.84%) with strong GFP signal was obtained with 25% of PEG4000 concentration for 10 min of transfection time. This result demonstrated that the transfection efficiency of cassava protoplasts was sufficiently high to be used for gene functional analysis in the future.

Using the optimized protocol that we developed, subcellular localization of MeSTP7 was investigated. Cassava MeSTP7 is a homologous gene of STP1 (SUGAR TRANSPORTER PROTEIN 1) in Arabidopsis thaliana and Nicotiana tabacum, which encodes an H+/monosaccharide cotransporter containing 12 putative transmembrane domains and is a putative plasma membrane protein [39–41]. Our results showed that MeSTP7 have been likely localized to the cassava plasma membrane. Similar to the localization in cassava protoplasts, the MeSTP7 protein was distributed in Arabidopsis plasma membrane using a heterologous transient gene expression system. This confirmed that the cassava mesophyll protoplast expression is a reliable procedure for subcellular protein localization.

Conclusions

In summary, a highly efficient protocol for cassava mesophyll protoplast isolation and PEG-mediated transient expression was obtained through optimization of the type and concentration of enzymes for protoplast preparation, and PEG4000 concentration and treatment time for transfection. The method reported here can thus be very useful for molecular and cellular studies in cassava, especially combined with genetics, genomics, transcriptomics and proteomics. It is noteworthy that the simple incubation buffers described in this article do not support division of mesophyll protoplasts. Therefore, the system will not appropriate to evaluate the expression of cassava genes involved in, e.g., cell cycle, plant dedifferentiation, or genes that require long-term follow-up (several cycles of cell division). The protoplast regeneration protocols developed by Shahin and Shephard [31], Sofiari et al [32] and Anthony et al [33] can be used for this purpose.

References

  1. Abel S, Theologis A. Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant J. 1994;5:421–7.

    Article  CAS  Google Scholar 

  2. Faraco M, Di Sansebastiano GP, Spelt K, Koes RE, Quattrocchio FM. One protoplast is not the other! Plant Physiol. 2011;156:474–8.

    Article  CAS  Google Scholar 

  3. Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007;2:1565–72.

    Article  CAS  Google Scholar 

  4. Sheen J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 2001;127:1466–75.

    Article  CAS  Google Scholar 

  5. Siritunga D, Arias-Garzon D, White W, Sayre R. Over-expression of hydroxynitrile lyase in transgenic cassava (Manihot esculenta Crantz) roots accelerates cyanogenesis. Plant Biotech J. 2004;2:37–43.

    Article  CAS  Google Scholar 

  6. Miao YS, Jiang LW. Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells. Nat Protoc. 2007;2:2348–53.

    Article  CAS  Google Scholar 

  7. De Caroli M, Lenucci MS, Manualdi F, Dalessandro G, De Lorenzo G, Piro G. Molecular dissection of Phaseolus vulgaris polygalacturonase-inhibiting protein 2 reveals the presence of hold/release domains affecting protein trafficking toward the cell wall. Front Plant Sci. 2015;6:660.

    Article  Google Scholar 

  8. Nanjareddy K, Arthikala MK, Blanco L, Arellano ES, Lara M. Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis. BMC Biotechnol. 2016;16:53.

    Article  Google Scholar 

  9. Chen SB, Tao LZ, Zeng LR, Vega-Sanchez M, Umemura KJ, Wang GL. A highly efficient transient protoplast system for analyzing defence gene expression and protein-protein interactions in rice. Mol Plant Pathol. 2006;7:417–27.

    Article  CAS  Google Scholar 

  10. Kim MJ, Baek K, Park CM. Optimization of conditions for transient Agrobacterium-mediated gene expression assays in Arabidopsis. Plant Cell Rep. 2009;28:1159–67.

    Article  CAS  Google Scholar 

  11. Walter M, Chaban C, Schütze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J. 2004;40:428–38.

    Article  CAS  Google Scholar 

  12. Guo J, Morrell-Falvey JL, Labbe JL, Muchero W, Kalluri UC, Tuskan GA, Chen J-G. Highly Efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. PLoS One. 2012;7, e44908.

    Article  CAS  Google Scholar 

  13. Guo Y, Song X, Zhao S, Lv J, Lu M. A transient gene expression system in Populus euphratica Oliv. protoplasts prepared from suspension cultured cells. Acta Physiol Plant. 2015;37:160.

    Article  Google Scholar 

  14. Tan BY, Xu M, Chen Y, Huang MR. Transient expression for functional gene analysis using Populus protoplasts. Plant Cell Tissue Org Cult. 2013;114:11–8.

    Article  CAS  Google Scholar 

  15. Huang H, Wang Z, Cheng J, Zhao W, Li X, Wang H, Zhang Z, Sui X. An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system. Sci Hortic. 2013;150:206–12.

    Article  CAS  Google Scholar 

  16. Bai Y, Han N, Wu J, Yang Y, Wang J, Zhu M, Bian H. A transient gene expression system using barley protoplasts to evaluate microRNAs for post-transcriptional regulation of their target genes. Plant Cell Tiss Organ Cult. 2014;119:211–9.

    Article  CAS  Google Scholar 

  17. Wang H, Wang W, Zhan J, Huang W, Xu H. An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes. Sci Hortic. 2015;191:82–9.

    Article  CAS  Google Scholar 

  18. Burris KP, Dlugosz EM, Collins AG, Stewart Jr CN, Lenaghan SC. Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.). Plant Cell Rep. 2016;35:693–704.

    Article  CAS  Google Scholar 

  19. Hong SY, Seo PJ, Cho SH, Park CM. Preparation of leaf mesophyll protoplasts for transient gene expression in Brachypodium distachyon. J Plant Biol. 2012;55:390–7.

    Article  CAS  Google Scholar 

  20. Shen J, Fu J, Ma J, Wang X, Gao C, Zhuang C, Wan J, Jiang L. Isolation, culture, and transient transformation of plant protoplasts. Curr Protoc Cell Biol. 2014;63:2.8.1–17.

    Article  Google Scholar 

  21. Alves AGC. Cassava botany and physiology. In: Hillocks RJ, Thresh JM, Bellotti AC, editors. Cassava biology, production and utilization. Wallingford: CABI; 2001. p. 67–90.

    Google Scholar 

  22. Jennings DL, Iglesias C. Breeding for crop improvement. In: Hillocks RJ, Thresh JM, Bellotti AC, editors. Cassava biology, production and utilization. Wallingford, CABI/Embrapa, Colombia. 2001. p. 149-65.

  23. Liu J, Zheng Q, Ma Q, Gadidasu KK, Zhang P. Cassava genetic transformation and its application in breeding. J Integr Plant Biol. 2011;53:552–69.

    Article  CAS  Google Scholar 

  24. Prochnik S, Marri PR, Desany B, Rabinowicz PD, Kodira C, Mohiuddin M, Rodriguez F, Fauquet C, Tohme J, Harkins T, Rokhsar DS, Rounsley S. The cassava genome: current progress, future directions. Trop Plant Biol. 2012;5:88–94.

    Article  CAS  Google Scholar 

  25. Zhang P, Legris G, Coulin P, Puonti-Kaerlas J. Production of stably transformed cassava plants via particle bombardment. Plant Cell Rep. 2000;19:939–45.

    Article  CAS  Google Scholar 

  26. Siritunga D, Sayre R. Generation of cyanogen-free transgenic cassava. Planta. 2003;217:367–73.

    Article  CAS  Google Scholar 

  27. Siritunga D, Sayre R. Engineering cyanogens in cassava. Plant Mol Biol. 2004;56:661–9.

    Article  CAS  Google Scholar 

  28. Zhang P, Puonti-Kaerlas J. PEG-mediated cassava transformation using positive and negative selection. Plant Cell Rep. 2000;19:1041–48.

    Article  CAS  Google Scholar 

  29. Nyaboga EN, Njiru JM, Tripathi L. Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14. Front Plant Sci. 2015;6:411.

    Article  Google Scholar 

  30. Zainuddin IM, Schlegel K, Gruissem W, Vanderschuren H. Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces. Plant Methods. 2012;8:24.

    Article  Google Scholar 

  31. Shahin EA, Shephard JF. Cassava mesophyll protoplasts: Isolation, proliferation, and shoot formation. Plant Sci Lett. 1980;17:459–65.

    Article  CAS  Google Scholar 

  32. Sofiari E, Raemakers CJJM, Bergervoet JEM, Jacobsen E, Visser RGF. Plant regeneration from protoplasts isolated from friable embryogenic callus of cassava. Plant Cell Rep. 1998;18:159–65.

    Article  CAS  Google Scholar 

  33. Anthony P, Davey MR, Power JB, Lowe KC. An improved protocol for the culture of cassava leaf protoplasts. Plant Cell Tiss Organ Cult. 1995;42:299–302.

    Article  CAS  Google Scholar 

  34. De Angeli A, Monachello D, Ephritikhine G, Frachisse JM, Thomine S, Gambale F, Barbier-Brygoo H. The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles. Nature. 2006;442:939–42.

    Article  Google Scholar 

  35. Kantharajah A, Dodd W. Factors that influence the yield and viability of cucumber (Cucumis sativus L.) cotyledon protoplasts. Aust J Bot. 1990;38:169–75.

    Article  Google Scholar 

  36. Davey MR, Anthony P, Power JB, Lowe KC. Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv. 2005;23:131–71.

    Article  CAS  Google Scholar 

  37. Zhu L, Wang B, Zhou J, Chen L, Dai C, Duan C. Protoplast isolation of callus in Echinacea augustifolia. Colloid Surface B. 2005;44:1–5.

    Article  CAS  Google Scholar 

  38. Raikar SV, Braun RH, Bryant C, Conner AJ, Christey MC. Efficient isolation, culture and regeneration of Lotus corniculatus protoplasts. Plant Biotechnol Rep. 2008;2:171–7.

    Article  Google Scholar 

  39. Cordoba E, Aceves-Zamudio DL, Hernández-Bernal AF, Ramos-Vega M, León P. Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana. J Exp Bot. 2015;66:147–59.

    Article  CAS  Google Scholar 

  40. Sauer N, Friedländer K, Gräml-Wicke U. Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. EMBO J. 1990;9:3045–50.

    CAS  Google Scholar 

  41. Sauer N, Stadler R. A sink-specific H+/monosaccharide co-transporter from Nicotiana tabacum: cloning and heterologous expression in baker's yeast. Plant J. 1993;4:601–10.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank the anonymous reviewers for their helpful comments on the manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (31660412), Cassava Molecular Breeding Technology Integration and Germplasm Innovation (2010CB126606-001) and Mechanisms of Sugar Metabolism, Barren-resistance and Disease Resistance in Cassava and Rubber Tree (ZXBJH-XK001-1630032016008).

Availability of data and materials

The datasets supporting the conclusions of this article are available from the corresponding author on reasonable request.

Authors’ contributions

L-JL, J-PL and K-ML designed and supervised the study. J-ZW carried out the experiments, analyzed the data and wrote the preliminary masuscript. QL and X-SG participated in experimental procedures. L-JL and J-PL prepared the final manuscript. The manuscript was read and approved by all the authors.

Competing interests

The authors declare that they have no competing interests.

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  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, Hainan Province, 570228, China

    Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Li-Juan Luo & Jin-Ping Liu

  2. The Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province, 571101, China

    Kai-Mian Li

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  4. Kai-Mian Li
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  5. Li-Juan Luo
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  6. Jin-Ping Liu
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Corresponding authors

Correspondence to Li-Juan Luo or Jin-Ping Liu.

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Wu, JZ., Liu, Q., Geng, XS. et al. Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz). BMC Biotechnol 17, 29 (2017). https://doi.org/10.1186/s12896-017-0349-2

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BMC Biotechnology

ISSN: 1472-6750

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