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Table 2 Genes cloned and efficiency of reconstitution

From: A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

Gene

Gene ID

Length (bp)

Vector

Positivea

yaiS

946967

558

pET22b

8 (7)

ybaY

945070

574

pET22b

8 (8)

nfnB

945778

654

pET22b

8 (6)

yohK

949125

696

pET22b

8 (8)

rlmB

948694

732

pET22b

8 (8)

nhoA

947251

846

pET22b

8 (7)

dapA

946952

879

pET22b

8 (8)

deoC

948902

780

pET22b

8 (6)

deoA

948901

1,230

pET22b

8 (5)

deoB

948910

1,224

pET22b

8 (8)

deoD

945654

721

pET22b

8 (7)

yjjJ

944883

1,332

pET22b

8 (8)

lplA

944865

1,017

pET22b

8 (8)

ytjB

946089

645

pET22b

8 (7)

serB

948913

969

pET22b

8 (8)

radA

948912

1,383

pET22b

8 (7)

yhdP

2847740

3,801

pET22b

8 (4)

yjjK

948909

1,668

pET22b

8 (8)

slt

948908

1,938

pET22b

8 (8)

yjiQ

948866

561

pET22b

8 (6)

yjiR

949089

1,413

pET22b

8 (8)

yjiS

948903

165

pET22b

8 (8)

yjiT

945056

1,503

pET22b

8 (6)

yjiV

2847669

2,937

pET22b

8 (7)

mcrC

948880

1,047

pET22b

8 (8)

mcrB

949122

1,380

pET22b

8 (6)

yjiW

7157066

399

pET22b

8 (8)

hsdS

948867

1,395

pET22b

8 (8)

hsdM

948872

1,590

pET22b

8 (8)

hsdR

948878

3,513

pET22b

8 (5)

mrr

948898

915

pET22b

8 (8)

yjiA

948882

957

pET22b

8 (8)

recB

947286

3,543

pET22b

8 (5)

yjiY

948914

2,151

pET22b

8 (8)

tsr

948884

1,656

pET22b

8 (8)

yjiZ

948879

1,362

pET22b

8 (7)

yjjM

7159433

915

pET22b

8 (8)

yjjN

7159438

1,023

pET22b

8 (8)

mdoB

7159439

2,292

pET22b

8 (4)

yeeJ

946498

7,077

pET22b

8 (6)

gasT

2520

306

pcDNA™ 3.1

8 (8)

mcm6

4175

2,466

pcDNA™ 3.1

8 (6)

slc18a2

6571

1,545

pcDNA™ 3.1

8 (7)

Gene cluster 1

 

10 kb

pET22b

8 (5)

Gene cluster 2

 

15 kb

pET22b

8 (6)

Gene cluster 3

 

20 kb

pET22b

8 (4)

  1. aNumber of colonies checked (number of positive colonies)