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Table 2 Genes cloned and efficiency of reconstitution

From: A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

Gene Gene ID Length (bp) Vector Positivea
yaiS 946967 558 pET22b 8 (7)
ybaY 945070 574 pET22b 8 (8)
nfnB 945778 654 pET22b 8 (6)
yohK 949125 696 pET22b 8 (8)
rlmB 948694 732 pET22b 8 (8)
nhoA 947251 846 pET22b 8 (7)
dapA 946952 879 pET22b 8 (8)
deoC 948902 780 pET22b 8 (6)
deoA 948901 1,230 pET22b 8 (5)
deoB 948910 1,224 pET22b 8 (8)
deoD 945654 721 pET22b 8 (7)
yjjJ 944883 1,332 pET22b 8 (8)
lplA 944865 1,017 pET22b 8 (8)
ytjB 946089 645 pET22b 8 (7)
serB 948913 969 pET22b 8 (8)
radA 948912 1,383 pET22b 8 (7)
yhdP 2847740 3,801 pET22b 8 (4)
yjjK 948909 1,668 pET22b 8 (8)
slt 948908 1,938 pET22b 8 (8)
yjiQ 948866 561 pET22b 8 (6)
yjiR 949089 1,413 pET22b 8 (8)
yjiS 948903 165 pET22b 8 (8)
yjiT 945056 1,503 pET22b 8 (6)
yjiV 2847669 2,937 pET22b 8 (7)
mcrC 948880 1,047 pET22b 8 (8)
mcrB 949122 1,380 pET22b 8 (6)
yjiW 7157066 399 pET22b 8 (8)
hsdS 948867 1,395 pET22b 8 (8)
hsdM 948872 1,590 pET22b 8 (8)
hsdR 948878 3,513 pET22b 8 (5)
mrr 948898 915 pET22b 8 (8)
yjiA 948882 957 pET22b 8 (8)
recB 947286 3,543 pET22b 8 (5)
yjiY 948914 2,151 pET22b 8 (8)
tsr 948884 1,656 pET22b 8 (8)
yjiZ 948879 1,362 pET22b 8 (7)
yjjM 7159433 915 pET22b 8 (8)
yjjN 7159438 1,023 pET22b 8 (8)
mdoB 7159439 2,292 pET22b 8 (4)
yeeJ 946498 7,077 pET22b 8 (6)
gasT 2520 306 pcDNA™ 3.1 8 (8)
mcm6 4175 2,466 pcDNA™ 3.1 8 (6)
slc18a2 6571 1,545 pcDNA™ 3.1 8 (7)
Gene cluster 1   10 kb pET22b 8 (5)
Gene cluster 2   15 kb pET22b 8 (6)
Gene cluster 3   20 kb pET22b 8 (4)
  1. aNumber of colonies checked (number of positive colonies)