Reaction 1 | Reaction 2a | Reaction 2b | |
---|---|---|---|
Template DNA | ~50 ng | ~500 ng | ~500 ng |
Forward primer (100 μM) | 0.25 μL | 0.25 μL | |
Reverse primer (100 μM) | 0.25 μL | 0.25 μL | |
Phusion GC Buffer (5×) | 10 μL | 10 μL | 10 μL |
dNTPs (10 mM) | 1 μL | 1 μL | 1 μL |
DMSO (100%) | 1.5 μL | 1.5 μL | 1.5 μL |
Phusion High Fidelity DNA Polymerase | 1 μL | 1 μL | 1 μL |
Add water to | 50 μL | 50 μL | 50 μL |