Fig. 4
OsbHLH59 and OsERF71 bind to OsXIP promoter in vitro and in vivo. a TFs (OsbHLH59, and OsERF71) bind to OsXIP promoter in yeast. Bait strain Y1HGold[pBait-AbAi] yeast cells was transformed with a prey vector, containing OsbHLH59 and OsERF71 fused to a GAL4 activation domain, respectively. Cells were grown in liquid media to OD600 of 0.1 (10−1) and diluted in a 10× dilution series (10−2 to 10−3). Of each dilution, 7 μl was spotted on media selecting for both plasmids (SD/−Leu) and selecting for interaction (SD/−Leu/AbA100), supplemented with 100 ng ml−1 AbA to suppress background growth. b ChIP-PCR analysis. The ChIP of TFs (OsbHLH59, and OsERF71) assays was performed using transgenic rice expressing the 35S:OsbHLH59-GFP fusion or 35S:OsERF71-GFP fusion. Products of ChIP assays were amplified using five specific primers (listed in Additional file 1: Table S1)