Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization

Table 3 Effect of calcium ions on recombinant AmyA1

Metal ions/chemical reagents	Concentration (mM)	Relative activity (%)^a
Blank^b	5	100 ± 1.0
EDTA^c	5	73.0 ± 1.8
EGTA^d	5	82.2 ± 3.5
Ca²⁺+ EDTA^e	5	81.9 ± 2.7
Ca²⁺+ EGTA^f	5	89.1 ± 3.8

^aThe data are the means of three independent experiments
^bThe activity of AmyA1 was determined in standard assay condition in the absent of metal ions
^cThe enzyme was dialyzed against 5 mM EDTA, then the activity was determined in standard assay condition
^dThe enzyme was dialyzed against 5 mM EGTA, then the activity was determined in standard assay condition
^eThe enzyme was dialyzed against 5 mM EDTA, then the activity was determined in the presence of 5 mM Ca²⁺
^fThe enzyme was dialyzed against 5 mM EGTA, then the activity was determined in the presence of 5 mM Ca²⁺

ISSN: 1472-6750