Table 2 Effect of different metal ions and chemical reagents on recombinant AmyA1
Metal ions/chemical reagents | Concentration (mM) | Relative activity (%)a |
|---|---|---|
Blank | 5 | 100 ± 3.7 |
Mg2+ | 5 | 117.1 ± 4.2 |
Co2+ | 5 | 91.1 ± 3.2 |
Mn2+ | 5 | 93.1 ± 3.1 |
Fe2+ | 5 | 95.9 ± 2.9 |
Cu2+ | 5 | 99.4 ± 5.3 |
Ni2+ | 5 | 61.3 ± 1.8 |
Ca2+ | 5 | 113.0 ± 4.8 |
Zn2+ | 5 | 100.7 ± 3.5 |
EDTA | 5 | 81.7 ± 2.1 |
Teween-80 | 0.5% (v/v) | 63.2 ± 1.5 |
Teween-20 | 0.5% (v/v) | 45.3 ± 1.7 |
Triton X-100 | 0.5% (v/v) | 58.8 ± 2.2 |
Methanol | 0.5% (v/v) | 53.2 ± 1.5 |
Isopropanol | 0.5% (v/v) | 85.0 ± 3.0 |
DMSO | 0.5% (v/v) | 81.3 ± 2.7 |