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Table 2 Cost/benefit comparison of lentiviral shRNA methods

From: A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector

Method

Cost

Unit

Benefits

Drawbacks

EZ-Tet-pLKO

$50

per shRNA

Low cost, Customizable, Puro/Hygro/Blast

Efficient 7 nt loop

2–3 day process

TRC library (non-inducible)

$208/$330

(glycerol stock)

per shRNA/Set of 3–6

No cloning required

Non-inducible, 6 nt loop

TRC library (Dox-inducible)

$450/$1100

(glycerol stock)

per shRNA/ Set of 3

No cloning required

High cost, 6 nt loop

$1195

(viral particles)

1 unique or 3 mixed

Ready to use