Table 2 Cost/benefit comparison of lentiviral shRNA methods
| Method | Cost | Unit | Benefits | Drawbacks |
|---|---|---|---|---|
| EZ-Tet-pLKO | $50 | per shRNA |
Low cost, Customizable, Puro/Hygro/Blast Efficient 7 nt loop | 2–3 day process |
| TRC library (non-inducible) |
$208/$330 (glycerol stock) | per shRNA/Set of 3–6 | No cloning required | Non-inducible, 6 nt loop |
| TRC library (Dox-inducible) |
$450/$1100 (glycerol stock) | per shRNA/ Set of 3 | No cloning required | High cost, 6 nt loop |
|
$1195 (viral particles) | 1 unique or 3 mixed | Ready to use |