Table 4 Activity of key enzymes involved in PMA and pullulan biosynthesis when A.pullulans HA-4D was grown in JAT medium or GM medium. PYC: pyruvate carboxylase; MDH: malate dehydrogenase; PGM: α-phosphoglucose mutase; UDPG: UDP-glucose pyrophosphorylase; FKS: glucosyltransferase
Time | Medium | Specific enzyme activities (U/mg protein) | ||||
|---|---|---|---|---|---|---|
PYC | MDH | PGM | UDPG | FKS | ||
40 h | GM | 0.18 ± 0.03 | 4.55 ± 0.44 | (7.86 ± 0.40) × 10−3 | (56.89 ± 5.88) × 10−3 | 0.79 ± 0.05 |
|  | JAT | 0.31 ± 0.02** | 5.37 ± 0.30 | (8.25 ± 0.19) × 10−3 | (53.41 ± 3.95) × 10−3 | 0.85 ± 0.03 |
100 h | GM | 0.10 ± 0.02 | 2.52 ± 0.43 | (6.29 ± 0.37) × 10−3 | (25.51 ± 3.35) × 10−3 | 0.68 ± 0.04 |
|  | JAT | 0.19 ± 0.03* | 2.88 ± 0.22 | (5.86 ± 0.42) × 10−3 | (27.86 ± 4.09) × 10−3 | 0.65 ± 0.05 |