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Table 2 Effects of different supplements on the co-production of PMA and pullulan by A.pullulans HA-4D in JAT fermentation medium. Shake flask fermentation was carried out at 25 °C and 200 rpm for 7 days

From: Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Components

Concentration

(g/L)

PMA

(g/L)

Pullulan

(g/L)

DCW

(g/L)

NaNO3

0

31.9 ± 0.5

5.3 ± 0.4

12.5 ± 0.3

1

33.2 ± 0.4*

6.3 ± 0.4

13.9 ± 0.4*

2

30.8 ± 0.4

5.8 ± 0.4

15.8 ± 0.5**

KH2PO4

0

34.0 ± 0.4

6.1 ± 0.5

12.8 ± 0.4

0.1

33.4 ± 0.4

6.3 ± 0.4

13.9 ± 0.9

0.2

32.8 ± 1.1

6.6 ± 0.5

14.3 ± 0.8

MgSO4

0

35.1 ± 0.3

5.8 ± 0.5

13.2 ± 0.5

0.1

34.1 ± 0.8

6.1 ± 0.3

12.9 ± 0.6

0.2

33.7 ± 0.9

6.7 ± 0.3

12.5 ± 0.4

KCl

0

36.3 ± 0.5

5.5 ± 0.4

11.9 ± 0.2

0.5

35.1 ± 0.5

5.7 ± 0.4

12.5 ± 0.4

1

34.6 ± 0.9

6.4 ± 0.3

12.9 ± 0.8

ZnSO4

0

36.8 ± 0.4

6.0 ± 0.3

12.5 ± 0.4

0.1

36.2 ± 0.6

5.5 ± 0.4

11.9 ± 0.4

0.2

35.8 ± 0.4

5.1 ± 0.5

11.6 ± 0.3

CaCO3

0

26.3 ± 0.4

9.7 ± 0.6

11.5 ± 0.4

15

33.9 ± 0.6**

6.8 ± 0.2**

11.9 ± 0.5

30

36.6 ± 0.7**

6.0 ± 0.4**

12.4 ± 0.9

  1. *0.01 < P < 0.05
  2. **P < 0.01