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Fig. 5 | BMC Biotechnology

Fig. 5

From: Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

Fig. 5

Time course and visual effect of decolorization of four dyes by the crude enzymatic solution. A1, B1, C1 and D1 show the in vitro decolorization rate of four dyes over time by consumption of crude rGluMnP1, which included Drimaren Blue CL-BR (A1), Drimaren Yellow X-8GN (B1), Drimaren Red K-4Bl (C1) and Disperse Navy Blue HGL (D1). The black line in each image shows the decolorization rate after treatment by rGluMnP1; the red line shows the results for the negative control. A2, B2, C2 and D2 showed the visual decolorization effect of four dyes by control (untransformed yeast) and crude rGluMnP1 solutions (transformed yeast). (a) and (b) show the visual decolorization effects before/after treated by the untransformed yeast, (c) and (d) show the visual decolorization effects before/after treated by the yeast transformants. Yeasts were broken to prepare the crude enzyme solutions. The reactions were carried out in a 2 mL EP tube

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