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Table 1 Strains used in this study

From: A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli

Strains

Genotype

Source

Suc-T110

E.coli ATCC#8739, ΔldhA, ΔpflB, ΔptsI, Ppck*-galP, Ppck*-pck

[41]

HX024

Suc-T110, ΔackA-pta, Ppck*-aceBA, Ppck*-dcuC, ΔmgsA,adaptively evolved for 1440 generations

[4]

RpoBD645Y

Suc-T110:: rpoB (D654Y)

In this study

AgaRR109W

Suc-T110:: agaR (R109W)

In this study

OV-LamB

Suc-T110, Ppck*- malK-lamB-malM

In this study

RpoBD645Y/∆malEFG

RpoBD645Y, ∆malE-malF-malG

In this study

RpoBD645Y/∆malEFGKM

lamB

RpoBD645Y, ∆malE-malF-malGmalK-lamB-malM

In this study

  1. Ppck* stand for a mutant of the E. coli pck promoter which with a G-to-A mutation at position -64 relative to the ATG start codon