Fig. 5From: A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli Schematic model illustrating how the D654Y mutation of RpoB affects the osmotolerance of E. coli via derepression of sugar transporter genes. Under osmotic stress, the Suc-T110 strain relies on the OmpC porin to transport glucose into the periplasm, which is then transported into the cytoplasm by GalP. However, this transportation process is inefficient. Under osmotic stress in RpoBD645Y, glucose enters cell via both OmpC and derepressed LamB, and then is transported into the cytoplasm by both GalP and derepressed maltose transporter. Transport into RpoBD645Y is more robust due to the presence of more porins and transporters involved in glucose uptakeBack to article page