Fig. 4

Malfunction of the maltose transporter decreased cell growth and succinate production under hyperosmotic conditions. Growth of (a) RpoBD645Y/ΔmalEFG and (d) RpoBD645Y/∆malEFGKM∆lamB under osmotic stress (12% w/v glucose). SA production by (b) RpoBD645Y/ΔmalEFG and (e) RpoBD645Y/∆malEFGKM∆lamB after 96 h of fermentation. Glucose consumption by (c) RpoBD645Y/ΔmalEFG and (f) RpoBD645Y/∆malEFGKM∆lamB were also measured. Strains RpoBD645Y and Suc-T110 were used as controls. Data are the mean with the standard error of the mean (SEM, n = 3). The significance of differences was calculated with one-way ANOVA; the asterisks indicate a significant difference from the controls (*** P < 0.001; ** P < 0.01; * P < 0.05; NS = not significant)