Fig. 2

Transcriptional response to osmotic stress in Suc-T110 and RpoBD645Y cells. a The altered gene expression patterns elicited by the rpoB mutation under either normal (left panel) or osmotic stress (right panel) conditions were separately plotted in as an MD-plot. M values (Y axis) represent the log2 normalized fold changes. D values (X axis) are the absolute differences in RPKM between Suc-T110 and RpoBD645Y. Each point represents a transcript with a detected expression level. Red or blue points represent differentially expressed genes with increased or decreased abundance, respectively (q value ≥ 0.90 and |log2 ratio| ≥ 1), whereas gray points are genes with no differences in expression. Transcripts used to interpret the osmotolerance phenotype are plotted in different colors. b Functional enrichment analysis of the upregulated genes. Typical FunCat terms are listed, and the asterisks (***) indicate a significant enrichment (unadjusted P-value < 0.001)