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Fig. 5 | BMC Biotechnology

Fig. 5

From: Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

Fig. 5

Reprogramming mechanism in the process of mRNA induction. a The expression of Oct4, Sox2, Klf4, and c-Myc were evaluated by qRT-PCR. Their expression reached the maximum 6 days after transfection and then decreased to the minimum on day 12. Then, the expression of these reprogramming factors increased gradually afterwards. b Expression of the endogenous pluripotent genes Nanog and TERT began to increase on day 12. c Western blot analysis of the OCT4, SOX2, KlLF4, C-MYC and NANOG during mRNA-induction process. d1, d6, d9, d12, d15, d18, d21 represent the day after exogenous mRNA transfection into GEFs. d Schematic diagram of the endogenous and exogenous expression pattern of the reprogramming markers during mRNA-induced GEFs reprogramming process

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BMC Biotechnology

ISSN: 1472-6750

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