Fig. 4

Goat iPSCs is able to differentiate into three germ layer cells. a Morphology of embryoid bodies derived from the goat iPS1 cell line. Scale bar: 50 μm. b qRT-PCR evaluation of the three germ layer marker genes expression was performed on the embryoid bodies derived from different generations of the goat iPS1 cell line. AFP and DCN (endoderm), NeuroD and NFH (ectoderm), Myf5 and Renin (mesoderm). The emboyid bodies derived express these marker genes. c Immunocytochemistry staining of the three germ layers markers, smooth muscle actin (SMA), Sox17, and βIII-tubulin (TUJ-1), were conducted, showing positive staining of the emboyid bodies induced from the goat iPSCs. iPSCs without induction were used as the blank control, which did not express the three markers. Immunofluorescence staining of the induced embyoid cells without primary antibodies was used as the negative control. Scale bar: 50 μm